Zhou Enmin
Zhou Enmin, MD, PhD Dean & Distinguished Professor, Immunobiology
College of Veterinary Medicine
Northwest A&F University
22 Xinong Road, Yanglin, Shaanxi Province, China 712100
Tel. 86-29-87091280
Fax.86-29-87091280
E-mail. zhouem@nwsuaf.edu.cn
EDUCATION:
MD. Medicine (Equivalent, 1982), Baotou Medical College, Baotou, China
PhD. Immunology (1993); Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
PROFESSIONAL EXPERIENCE:
7/2010- present Dean & Distinguished Professor, Immunobiology
College of Vet erinary Medicine
Northwest A&F University
7/2007–6/2010 “Taishan Scholar” and Distinguished Professor
Associate Dearn, College of Veterinary Medicine, Shandong Agricultural University, TaiAn, Shandong Province, China.
5/2006–7/2007 “Longjiang Scholar” and Distinguished Professor
Department of Preventive Veterinary Medicine, College of Animal Science and Technology, BaYi Agricultural University, Daqing, Heilongjiang Province, China.
6/2000– 5/2006 Head, Serology Section, Veterinary Diagnostic Laboratory, Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa.
12/2002- 12/2005 Adjunct Professor, Department of Veterinary Microbiology, College of Veterinary Medicine, Northeast Agriculture University, Harbin, China.
9/1998-6/2000 Adjunct Associate Professor, Department of Animal Science, University of Manitoba.
4/1996-5/2000 Senior Research Scientist and Head of Immunodiagnostic Laboratory, National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada.
7/1992- 3/1996 Research Scientist, Virology Section, Animal Diseases Research Institute, Agriculture Canada, Nepean, Ontario, Canada.
1/1995-12/1998 Adjunct Assistant Professor, Department of Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada.
9/1985-8/1988 Visiting Scientist and Postdoctoral Fellow, Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas, USA.
9/1982-8/1985 Assistant Professor, Department of Medical Microbiology, Baotou Medical College, Baotou, China.
HONOR and AWARDS:
2009 The Second One-Hundred Oversee Chinese Outstanding Achievement Award
2008 Chair person, Section of PRRS: Disease Situation, Epidemiology, Diagnosis, Prevention and Control, On-Going and Future Research. Workshop: Research in Swine Viral Diseases “Building Bridges”, organized by EPIZONE, European Commission and Shanghai Veterinary Research Institute, Shanghai 7-9 July 2008.
2007 Chair person, Section of Viral Diseases; 15th World Veterinary Poultry Congress, 10-15 September 2007.
2006 Natural Science Achieve Award, “Research in Avian hepatitis E virus epitope mapping and vaccine”, Daqing city, Heilongjiang Province.
2003 Invited to organize a workshop on Quality Control Issues in Animal Disease Diagnostic Testing, by Ministry of Agriculture of China, Qingdao, China.
2002 Invited to organize a workshop on Veterinary Diagnostic Immunology by North East Agricultural University, Harbin, China.
2001 Organized a workshop on Porcine Reproductive and Respiratory Syndrome Immunology: Diagnosis and Prevention. China Agricultural University, Beijing, China.
1998 Internship from Institute Interamericain De Cooperation Pour L’ Agriculture, Bureau Au Canada to validate the C-ELISA tests for BTV and EHD in Paraguay and to establish the increased exchange of information on issues important for facilitating trade.
1988 Chaired the Immunodiagnostic Session at the 5th International Veterinary Immunology Symposium, Ludhiana, India.
1988 Internship from Institute Interamericain De Cooperation Pour L’ Agriculture, Bureau Au Canada to validate the C-ELISA tests for BTV in Argentina and to establish the increased exchange of information on issues important for facilitating trade.
1988 Chaired the session of Novel Vaccination Approaches, IBC International Symposium of Veterinary Vaccines, Bethesda, MD.
1988 Chaired the seminar on Anti-idiotype and Immunity, National Quarantine Institute, Chinese Ministry of Agriculture, Qingdao, China.
1993 Shering Travel Grant Award for the 48th Annual Meeting of American Academy of Allergy and Immunology, Orlando, Florida, USA, March 1993.
1992 Postdoctoral Fellowship Award from Manitoba Health Research Council, Manitoba, Canada.
1992 Travel Grant Award from Canadian Society of Immunology to attend the 8th International Congress of Immunology, Budapest, Hungary.
1992 Travel Grant Award from Canadian Society of Immunology to attend the Spring Meeting of Canadian Society of Immunology, Montreal, Canada
1991 Schering Travel Grant Award for the 47th Annual Meeting of American Academy of Allergy and Immunology, San Francisco, CA.
1990 Student Research Award of Manitoba Medical Service Foundation for Excellence in Research, Manitoba, Canada.
1990 Schering Travel Grant Award for the 46th Annual Meeting of American Academy of Allergy and Immunology, Maryland, USA, March 1990.
1987 Ad Hoc Reviewer for the Viral Immunology, published by Mary Ann Lieber, Inc., publishers, USA.
1984 Excellent Teacher Award from Baotou Medical College, Baotou, China.
Current Research Areas:
PRRSV and host interaction and PRRSV immune regulation:
Funding: PRRSV and host interaction: China NSFC, 2008-2010,$350,000.
Major swine disease prevention and control: Shandong Major Research Application Grant, 2008-2010, $2,000,000.
Avian hepatitis E virus zoonosis and epitope mapping;
Funding: “Taishan Scholar” research funds 2007-2012,$2,000,000.
SCHOLARDHIP:
REFERRED PUBLICATIONS (TOTAL OF 65, *Corresponding author):
1. Qin Zhao, En-Min Zhou*, Shi-Wei Dong, Hong-Kai Qiu, Lu Zhang, Shou-Bin Hu, Fei-Fei Zhao, Shi-Jin Jiang, and Ya-Ni Sun. Genetic identification and sequence analysis of avian hepatitis E virus from chickens in China. Emerging and Infectious Diseases. In press, 2010.
2. Qin Zhao, En-Min Zhou*, Peiming Sun, Shiwei Dong, Lu Zhang, Yani Sun, and Shijin Jiang. Detection and analysis of avian hepatitis E virus in chicken flocks of China. Chinese J Pre Vet Med. In press, 2010.
3. Shao-Ning Liu, Xing-Xiao Zhang, Jin-Feng Zou, Zhi-Jing Xie, Yan-Li Zhu, Qin Zhao, En-Min Zhou, Shi-Jin Jiang. Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks. Vet Microbiol. 2010, online March 17
4. Zhao Q, Sun YN, Zhang XX, Kong YB, Xie ZJ, Zhu YL, Zhou EM, Jiang SJ. Evaluation of two experimental infection models for Avibacterium paragallinarum. Vet Microbiol. 2009. Aug 8. [Epub ahead of print]
5. Li XJ, Zhou EM*, Zhao Q, Xiao YH, Ma LH. Isolation and sequence analysis of a porcine reproductive and respiratory syndrom virus isolate. Southwest China Journal of Agricultural Sciences. 2009; 22(4):1103-1109.
6. Zhang HX, Jing ST, Zhou EM*, Sun PM, Dong SW, Li YJ. Detection and analysis of specific anti-avian hepatitis E virus antibodies in human sera. Chin J Health Lab Technol. 2009,19(8):1741-1744.
7. Ran XH, Wen XB, Meng F, Zhou EM. Expression of porcine parvovirus virus NS1 major antigen epitope protein and development of an indirect ELISA. Chinese J Vet Sci。2009,29(8):
8. Zhou E-M*, Jing ST, Peng J, Sun PM, Zhang L and Dong SW. Potential zoonosis of avian hepatitis E virus. China Poultry. 2009,31(1):5-9.
9. Zhou E-M*, Jing ST, Zhang L, Dong SW, Peng J and Sun PM. Current research progress for avian hepatitis E virus. Shandong J. Animal Sci. Vet. Med. 2008,11:1-3.
10. Zhang L, Zhou E-M*, Cui ZZ, Dong SW, Jing ST, Peng J, Sun PM, Sun SH and Qin ZM. Serological investigation of avian hepatitis E virus (HEV) and relationship between avian HEV and big liver and spleen syndrome. China Poultry. 2008,30(20):22-24.
11. Zhou E-M*, Xiao YH, Shi YF, Li XJ, Ma LH and Jing ST. Generation of internal image monoclonal anti-idiotypic antibodies against idiotypic antibodies to GP5 antigen of porcine reproductive and respiratory syndrome virus. J Virol Methods. 2008,149:300-308.
12. Zhou E-M, Guo H, Huang FF, Sun ZF and Meng XJ. Identification of two neutralization epitopes on the capsid protein 1 of avian hepatitis E virus (avian HEV). J. Gen. Virol. 2008,89:500-508.
13. Boyer TC, Ward MP, Wallace RL, Zhou E-M, Singer RS. Exploratory spatial data analysis of regional seroprevalence of antibodies against epizootic hemorrhagic disease virus in cattle from Illinois and Indiana. Ame J Vet Res. 2008,69(10):1286-1293.
14. Guo H, Zhou E-M*, Sun ZF and Meng XJ. Egg whites from eggs of chickens experimentally infected with avian hepatitis E virus contain infectious virus but evidence of complete vertical transmission is lacking. J. Gen. Virol. 2007,88:1532-1537.
15. Guo H, Zhou E-M*, Sun ZF and Meng XJ. Protection of chickens against avian hepatitis E virus (avian HEV) infection by immunization with recombinant avian HEV capsid protein. Vaccine. 2007,25:2892-2899.
16. Guo H, Zhou E-M,* Sun ZF, Meng XJ and Halbur PG. Identification of B-cell epitopes in the capsid protein of avian hepatitis E virus (avian HEV) that are common to human and swine HEVs or unique to avian HEV. J. Gen. Virol. 2006,87(1):217-223.
17. Trampel DW, Zhou E-M, Yoon KJ and Koehler KJ. Detection of antibodies in serum and egg yolk following infection of chickens with an H6N2 avian influenza virus. J. Vet. Diagn. Invest. 2006,18(5):437-442.
18. Opriessnig T, McKeown NE, Zhou E-M, Meng XJ and Halbur PG. Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-associated lesions provides evidence for differences in virulence. J. Gen. Virol. 2006,87(Pt 10):2923-2932.
19. Ameri-Mahabadi M and Zhou E-M. Idiotype and anti-idiotype: a review. Comp. Clin. Pathol. 2006,14:171-178.
20. Ameri-Mahabadi M, Zhou E-M* and Hsu W. A Western blot immunoassay as a confirmatory test for the presence of anti-mycoplasma hyopneumoniae antibodies in swine serum. J. Vet. Diagn. Invest. 2006,18(2):198-201.
21. Ameri-Mahabadi M, Zhou E-M* and Hsu W. Comparison of two swine mycoplasma hyopneumoniae enzyme-linked Immunosorbent assays for detection of antibodies from vaccinated pigs and field serum samples. J. Vet. Diagn. Invest. 2005,17: 61-64.
22. Clavijo A, Zhou E-M, Hole K, Galic B and Kitching P. Detection of antibodies against foot-and-mouth disease virus in a solid-phase competitive ELISA using a biotinylated 3ABC recombinant protein. J. Virol. Methods. 2004,15:217-227.
23. Zhou E-M*, Clavijo A, Jiang Z, Ameri-Mahabadi M and Zimmerman JJ. Induction of auto-anti-Idiotypic antibodies specific for antibodies to matrix and envelope glycoprotein from pigs experimentally infected with porcine reproductive and respiratory syndrome virus. Vet. Immunol. Immunopathol. 2004,101:49-59.
24. Skibbe D, Zhou E-M* and Janke BH. Comparison of a commercial ELISA with hemagglutination inhibition assay for serdiagnosis of swine influenza virus (H1N1) infection. J. Vet. Diagn. Invest. 2004,16:86-89.
25. Zhou J-Y, Chen Q-X, Ye J-X, Zhu J-X, Zheng X-J, Shang S-B, Chen T-F, Mu A-X and Zhou E-M. Serological investigation of porcine circovirus 2 infection. Chinese J. Vet. Med. 2004,24:1-3.
26. Zhou J-Y, Wu J-X, Cheng L-Q, Zheng X-J, Gong H, Shang S-B and Zhou E-M*. Expression of immunogenic S1 glycoprotein of infectious bronchitis virus in transgenic potatoes. J. Virol. 2003,77(16):9090-9093.
27. Jiang ZJ, Zhou E-M*, Ameri-Mahabadi M, Zimmerman JJ and Platt KB. Identification and characterization of auto-anti-idiotypic antibodies specific for antibodies against porcine reproductive and respiratory syndrome virus envelope glycoprotein. Vet. Immunol. Immunopathol. 2003,92:125-135.
28. Clavijo A, Tresnan DB, Jolie R and Zhou E-M. Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus. Can. J. Vet. Res. 2002,66:117-121.
29. Zhou E-M*, Ridd D, Riva J, Fernando L and Clavijo A. Development and evaluation of an IgM-capture ELISA for detection of recent infection with bluetongue virus in cattle. J. Virol. Methods. 2001,91:175-182.
30. Zhou E-M*, Riva J and Clavijo A. Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus. Clin. Diagn. Lab. Immunol. 2001,8:475-481.
31. Clavijo A, Lin M, Riva J and Zhou E-M. Application of competitive enzyme-linked immunosorbent assay for the serological diagnosis of classical swine fever virus infection. J. Vet. Diagn. Invest. 2001,13:357-360.
32. Clavijo A, Lin M, Riva J, Mallory M, Lin F and Zhou E-M. Development of a competitive ELISA using a truncated E2 recombinant protein as antigen for detection of antibodies to classical swine fever virus. Res. Vet. Sci. 2001,70:1-7.
33. Clavijo A, Riva J, Copps J, Robinson Y and Zhou E-M. Assessment of the pathogenicity of an emu-origin influenza A H5 virus in ostriches (Struthio camelus). Avian Pathol. 2001,30:83-89.
34. Pasick J, Handel, K, Zhou E-M, Clavijo A, Coates J, Robinson Y and Lincoln B. Incursion of epizootic hemorrhagic disease into the Okanogan Valley, British Columbia in 1999. Can. Vet. J. 2001,42:207-209.
35. Clavijo A, Munroe F, Zhou E-M, Booth TF and Roblesky K. Incursion of bluetongue into the Okanogan Valley, British Columbia. Can. Vet. J. 2000,41:312-314.
36. Heckert A, McIsaac M., Chan M and Zhou E-M. Experimental infection of emus (Dromaius novaehollandiae) with avian influenza virus of various virulence: clinical signs, virus shedding and serology. Avian Pathol. 1999,23:13-16.
37. Zhou E-M* and Afshar A. Characterization of monoclonal antibodies to epizootic hemorrhagic disease of deer virus (EHDV) and bluetongue virus by immunization of mice with EHDV recombinant VP7 antigen. Res. Vet. Sci. 1999,66:247-252.
38. Zhou E-M.* Anti-idiotype technique: an alternative approach for immunodiagnosis of bluetongue. Vet. Immunol. Immunopathol. 1999,72:237-242.
39. Wright P and Zhou E-M. Developments in international standardization. Vet. Immunol. Immunopathol. 1999,72:243-248.
40. Zhou E-M*, Chan M, Heckert RA, Riva J and Cantin M-F. Evaluation of a competitive ELISA for detection of antibodies against avian influenza virus nucleoprotein. Avian Dis. 1998,42:517-522.
41. Clavijo A, Zhou E-M, Vydelingum S and Heckert R. Development and evaluation of a novel antigen capture assay for the detection of classical swine fever virus antigens. Vet. Microbiol. 1998,60:155-168.
42. Zhou E-M*, Chan M, McIsaac M and Heckert R. Evaluation of antibody responses of emus (Dromaius novaehollandiae) to avian influenza virus infection. Avian Dis. 1998,42:757-761.
43. Xu G, Wilson W, Mecham J, Murphy K, Zhou E-M and Tabachnick W. VP7: an attachment protein of bluetongue virus for cellular receptors in Culicoides variipennes. J. Gen. Virol. 1997,78:1617-1623.
44. Prud'homme I, Zhou E-M, Traykova M, Trotter H, Chan M, Afshar A and Harding MJ. Production of a baculovirus-derived gp50 protein and utilization in a competitive enzyme-linked immunosorbent assay for the serodiagnosis of pseudorabies virus. Can. J. Vet. Res. 1997,61:286-291.
45. Zhou E-M* and Chan MKH. Immunoblot assay as a confirmatory test of competitive ELISA for serodiagnosis of bluetongue. J. Vet. Diagn. Invest. 1996,8(3):365-367.
46. Lin M and Zhou E-M*. 1996. Induction of antibodies to the bluetongue virus core polypeptide VP7 in sheep by internal image rabbit anti-idiotypic antibodies. Viral. Immunol, 9(1):35-43.
47. Lin M and Zhou E-M*. Evidence that two distinct populations of rabbit anti-idiotypic antibodies induced by three monoclonal antibodies specific for bluetongue virus core protein VP7. Microbiol. Immunol. 1996,40(4):295-302.
48. Lin M and Zhou E-M*. Biological mimicry of the bluetongue virus core protein VP7 by rabbit anti-idiotype. Microbiol. Immunol. 1996,40(6):435-441.
49. Zhou E-M, Kisil FT. Regulation of antibody formation to Ryegrass pollen allergen Lol pIV by an internal image anti-idiotypic monoclonal antibody. Immuno. 1995,84:343-349.
50. Zhou E-M* and Huang W. Anti-idiotypic antibody as a potential serodiagnostic reagent detecting bluetongue virus infection. J. Clin. Microbiol. 1995,33(4):850-854.
51. Zhou E-M* and Afshar A. Comparison of Freund's adjuvant and TiterMaxTM in inducing anti-idiotype to idiotypic antibodies against pseudorabies virus antigens. Vet. Immunol. Immunopathol. 1995,48:113-122.
52. Lin M and Zhou E-M*. Internal image anti-idiotypic antibody detects sheep antibodies to the bluetongue virus core protein VP7. Immunotechnol. 1995,1:151-155.
53. Zhou E-M* and Kisil FT. Antibody responses to allergen Lol pIV are suppressed following adoptive transfer of B lymphocytes from the internal image anti-idiotypic antibody treated mice. Cell. Immunol. 1995,165:77-83.
54. Zhou E-M*, Afshar A, Heckert RA and Nielsen K. Anti-idiotypic antibodies generated by sequential immunization detect the shared audiotape on antibodies to pseudorabies virus antigens. J. Virol. Methods. 1994,48:301-313.
55. Zhou E-M, Dzuba-Fisher JMM, Rector ES, Sehon AH and Kisil FT. A mouse monoclonal anti-idiotypic antibody detects a common idiotope on human, mouse, and rabbit antibodies to allergen Lol pIV. Scand. J. Immunol. 1991,34:307-316.
56. Zhou E-M, Lohman KL and Kennedy RC. Administration of noninternal image monoclonal anti-idiotypic antibodies induces idiotype-restricted responses specific for human immunodeficiency virus envelope glycoprotein epitopes. Virol. 1990,174:9-17.
57. Zhou E-M and Kennedy RC. Strategies for developing vaccines against acquired immunodeficiency syndrome. Biochem. Biophysica. Acta Cancer Res. Suppl. 1989,986:301-313.
58. Zhou E-M*, Wang D-R and Kennedy RC. Anti-idiotypic antibodies as potential candidates of vaccines. Chinese J. Immunol. 1988,2:70-73.
59. Zhou E-M, Dreesman GR, Kennedy RC. Anti-idiotypic antibodies: A new generation of vaccines against infectious agents. Microbiol. Sci. 1987,4:36-40.
60. Kennedy RC, Zhou E-M, Lanford RE, Chanh TC and Bona CA. Possible role of anti-idiotypic antibodies in the induction of tumor immunity. J. Clin. Invest. 1987,80:1217-1224.
61. Zhou E-M, Chanh TC, Dreesman GR, Kanda P and Kennedy RC. Immune response to human immunodeficiency virus: In vivo administration of anti-idiotype induces an anti-gp160 response specific for a synthetic peptide. J. Immunol. 1987,139:2950-2956.
62. Ren Q, Jin Z-C, Zhou E-M. Influence of uniform intensity magnetic field on the antibody forming cells in mice. J. Inner Mongolia Med. 1981,1(3):146-149. }
PETENT:
Zhou E-M. A novel PRRS virus receptor and the receptor blocking agents. China patent #200910013683.3. 2009.
Hsu WH, Young TF, Ross RF and Zhou E-M. Mycoplasma Polypeptide. US and International Filing date: April 4, 2003.
BOOK CHAPTERS:
63. Lowe DB, Shearer MH, Jumper CA, Zhou E-M and Kennedy. 2008. Plasmid DNA as prophylactic and therapeutic vaccines for cancer and infectious diseases. In Plasmids: Current Research and Future Trends (Georg Lipps ed.), Horizon Scientific Press.
64. Zhou E-M and Lin M. 1997. Anti-idiotype to bluetongue virus VP7 antigen - potential candidate of diagnostic reagent and vaccine. in Idiotypes in Medicine - Infections, Autoimmunity and Cancer (Y. Shoenfeld, R.C. Kennedy and T. Ferrone ed.), p347-355. Elsevier Science, The Netherlands.
65. Dreesman GR, Henkel RD, Zhou E-M and Kennedy RC. 1988. Properties of a protective anti-idiotype vaccine for viral hepatitis B. In Vaccine: New concepts and Developments (H. Kohler and P.T. LoVerde, eds.) Longman Group Ltd., UK.
PROCEEDINGS:
1. Zhou E-M*, Xiao YH, Wang WW, Ma LH, Li XJ, Shi YF. A potential novel PRRSV receptor on MA-104 and porcine alveolar macrophages. Workshop: Research in Swine Viral Diseases, “Building bridges” Shanghai, p(suppl) 10.
2. Zhou E-M*, Cui ZZ, Zhang L, Dong SW, Jing ST, Peng J, Sun PM and Qin ZM. 2008. Serological investigation of avian hepatitis E virus (HEV) and relationship between avian HEV and big liver and spleen syndrome. Chinese Association for Animal Science and Veterinary Medicine, The 14th Symposium of Avian Diseases, p 44.
3. Zhou E-M*, Cui YS, Xiao YH, Shi YF, Wang WW and Liu Y. 2008. Characterization of a novel PRRSV receptor. Chinese Association for Animal Science and Veterinary Medicine, The 3rd Swine Diseases Control and Prevention Symposium, p195-196.
4. Zhou E-M*, Guo HL, Peng J, Jing ST, Sun PM, Dong SW, Zhang L, Cui YS, Jiang SJ, Chang WS and Cheng ZQ. 2008. Current research in avian hepatitis E virus immunology. Chinese Association for Animal Science and Veterinary Medicine, The 1st Veterinary Publish Health Symposium, p75-79.
5. Zhou E-M*, Guo H. 2007. Avian Hepatitis E Virus (Avian HEV) Immunity: Antigenic Epitope Mapping of Avian HEV ORF2 Protein That Are Unique to Avian HEV or Common to Human and Swine HEVs. XV Congress of the World Veterinary Poultry Association, p615.
6. Zhou E-M,* Zimmerman JJ, Simer R. 2005. Novel approaches for PRRS diagnosis: identification of PRRS antibodies in saliva and meat juice. The 13th Swine Disease Conference, p14.
7. Zhou E-M,* Qin QS, Zimmerman JJ. 2005. Identification and characterization of a putative cellular receptor for porcine reproductive and respiratory syndrome virus. The 2005 International PRRS Symposium and Conference of Research Workers in Animal Diseases, p140.
8. Zhou E-M,* Zimmerman JJ, Simer R. 2005. Detection of isotype-specific anti-PRRSV antibodies in swine saliva samples. The 2005 International PRRS Symposium and Conference of Research Workers in Animal Diseases, p139.
9. Guo HL, Meng X-J, Zhou E-M. 2005. Recombinant ORF2 protein of avian hepatitis E virus (HEV) provided protection of chickens against avian HEV infection. North Central American Association for Microbiologists, p245.
10. Trampel DW, Zhou E-M, Yoon K-J. 2005. Detection of antibodies in serum and eggs & virus shedding in eggs following infection with an H6N2 avian influenza virus. American Veterinary Medical Association Annual Meeting, p367.
11. Ameri-Mahabadi M, Zhou E-M and Jang Z. 2005. The mouse as an experimental model to modulate antibody response to porcine reproductive and respiratory syndrome virus glycoprotein 5 using anti-idiotypic antibodies. J Vet Pathol 42:5, p. 707.
12. Zhou E-M, Ameri-Mahabadi M, Jiang J and Zimmerman JJ. 2004. Anti-idiotype induced reduction of PRRS virus infection. The 18th International Pig Veterinary Society Congress, p359.
13. Ameri-Mahabadi M and Zhou E-M. 2003. Western blot immunoassay as a confirmatory test for ELISAs to detect antibodies against Mycoplasma hyopneumoniae. American Association of Veterinary Diagnosticians, p52.
14. Ameri-Mahabadi and Zhou E-M. 2002. Serodiagnosis of swine Actinobacillus pleuropneuromoniae: evaluation of an indirect ELISA in comparison with complement fixation test. American Association of Veterinary Diagnosticians, p40.
15. Zhou E-M, Zimmerman JJ, Zhou XK, Platt KB, Yoon K-J and Horter D. 2002. Development of a blocking ELISA for detection of swine antibodies to PRRSV. American Association of Veterinary Diagnosticians, p60.
16. Zhou E-M, Zimmerman JJ, Zhou KX, Jiang Z, and Platt KB. 2002. IgM-capture ELISA as a serodiagnostic test for detection of primary infection of PRRSV in pigs. American Association of Swine Veterinarians, p 295-298.
17. Zhou E-M, Jiang ZJ and M Ameri-Mahabadi. 2002. Auto-anti-idiotype and PRRS virus infection. The 17th International Pig Veterinary Society, p 246.
18. Zhou E-M, Jiang Z, and Zimmerman JJ. 2002. Auto-anti-idiotype: its potential regulation of porcine reproductive and respiratory syndrome virus infection. The FASEB Journal, 518.15.
19. Zhou E-M. 2002. Serodiagnosis of swine Mycoplasma hyopneumoniae: evaluation of current commercial ELISA tests and development of alternative serologic assays. The 14th International Congress of the International Organization for Mycoplasmology (IOM), p80.
20. Zhou E-M,* Chan M, Heckert RA, Riva J, and Cantin M-F. 1998. A Competitive Enzyme-Linked Immunosorbent Assay for Avian Influenza Serological Surveillance. Fourth International Symposium on Avian Influenza. Am. Asso. Avian Pathol. P 305-312, 1998.
21. Kennedy RC, Zhou E-M, Dreesman GR. 1986. Anti-idiotypic antibodies modulate the immune response to hepatitis B surface antigen. IXth International Congress of Infectious and Parasitic Diseases (W. Marget, W. Lang, E. Gabler-Sandgerg, eds.), volume I, p 49-51.
ABSTRACTS:
Invited:
1. Zhou E-M*, Xiao YH, Wang WW, Ma LH, Li XJ, Shi YF. A potential novel PRRSV receptor on MA-104 and porcine alveolar macrophages. Workshop: Research in Swine Viral Diseases, “Building bridges”, Shanghai China, July 7-9, 2008.
2. Zhou E-M*, Cui ZZ, Zhang L, Dong SW, Jing ST, Peng J, Sun PM and Qin ZM. 2008. Serological investigation of avian hepatitis E virus (HEV) and relationship between avian HEV and big liver and spleen syndrome. Chinese Association for Animal Science and Veterinary Medicine, The 14th Symposium of Avian Diseases, Yangzhou China, Sept. 25-29, 2008.
3. Zhou E-M*, Cui YS, Xiao YH, Shi YF, Wang WW and Liu Y. 2008. Characterization of a novel PRRSV receptor. Chinese Association for Animal Science and Veterinary Medicine, The 3rd Swine Diseases Control and Prevention Symposium, Dalian China, Sept. 22-24, 2008.
4. Zhou E-M*, Guo HL, Peng J, Jing ST, Sun PM, Dong SW, Zhang L, Cui YS, Jiang SJ, Chang WS and Cheng ZQ. 2008. Current research in avian hepatitis E virus immunology. Chinese Association for Animal Science and Veterinary Medicine, The 1st Veterinary Publish Health Symposium, Shanghai China, May 22-24, 2008.
5. Zhou E-M*, Zimmerman JJ, Simer R. Novel approaches for PRRS diagnosis: identification of PRRS antibodies in saliva and meat juice. The 13th Swine Disease Conference, Ames, IA, November 2005.
6. Zhou E-M*. Anti-idiotypic antibodies as a novel diagnostic reagent and potential vaccine for porcine reproductive and respiratory syndrome virus. National Quarantine Institute, Ministry of Agriculture, Qingdao, China, June 2003.
7. Zhou E-M*. Immune modulation of PRRS virus infection and development of a novel diagnostic test for Mhyo antibody detection. Iowa Pork Congress, Des Moines, Iowa, January 2003.
8. Zhou E-M*, Jiang Z, Ameri-Mahabadi M, Zimmerman JJ. Improve Immunodiagnostic Sensitivity and Specificity of PRRS Virus Infection and Identify Possible Potential Virus Carriers Using the Combined Immunological Assays. 34th American Association of Swine Veterinarians Annual Meeting, Kissimmee, Florida, March 8-11, 2003.
9. Zhou E-M*. How to Determine False Positive Reactions of PRRS ELISA. The 11th Iowa State University Swine Disease Conference, Ames, Iowa, November 2002.
10. Zhou E-M*, Zimmerman JJ, Zhou KX, Jiang Z, Chang CC, Platt KB. IgM-capture ELISA as a serodiagnostic test for detection of primary infection of PRRSV in pigs. 33rd Annual Meeting of American Association of Swine Veterinarians, Kansas City, Missouri, March 2-5, 2002.
11. Zhou E-M*, Afshar A, Huang W, Gong X and Wilson W. Anti-idiotype technique: an alternative approach for immunodiagnosis. Fifth International Veterinary Symposium, Ludhiana, India, Nov. 8-13, 1998.
12. Wright P, Zhou -M. Development in international standardization. Fifth International Veterinary Symposium, Ludhiana, India, Nov. 8-13, 1998.
13. Zhou E-M*. Competitive ELISA for Avian Influenza Serologic Surveillance. Fourth International Symposium on Avian Influenza, Athens, Georgia, USA, May 28-31, 1997.
14. Zhou E-M*. Anti-idiotypic antibodies as potential candidates of vaccines and serodiagnostic reagents to infectious diseases. The IBC International Symposium of Veterinary Vaccines. Bethesda, MD, October 27-28, 1994.
15. Zhou E-M*. Generation of the internal image anti-idiotypic antibodies to pseudorabies virus antigens. Abstract to ADRI, Nepean Seminar Committee, May 27, 1993.
16. Zhou E-M*. Anti-idiotype: Their potential in serological testing for foreign animal diseases. The 43rd Conference of Canadian Laboratory Workers in Animal Diseases, ADRI, Nepean, June 1-3, 1993.
Non- invited:
1. Zhou E-M*, Qin QS and Zimmerman JJ. Ientification and characterization of a putative cellular receptor for porcine reproductive and respiratory syndrome virus. The 2005 International PRRS Symposium and Conference of Research Workers in Animal Diseases. St. Louis, MO, December 2-6, 2005.
2. Zhou E-M*, Zimmerman JJ and Simer R. Detection of isotype-specific anti-PRRSV antibodies in swine saliva samples. The 2005 International PRRS Symposium and Conference of Research Workers in Animal Diseases. St. Louis, MO, December 2-6, 2005.
3. Trampel DW, Zhou E-M and Yoon K-J. Detection of antibodies in serum and eggs & virus shedding in eggs following infection with an H6N2 avian influenza virus. American Veterinary Medical Association Annual Meeting, Minneapolis, MN, July 16-20, 2005.
4. Zhou E-M*, Ameri-Mahabadi M, Jiang ZJ and Zimmerman JJ. Anti-idiotype induced reduction of PRRS virus infection. The 18th Congress of the International Pig Veterinary Society, Hamburg, Germany, June 2004.
5. Guo H, Zhou E-M*, Sun ZF and Meng XJ. Species specific and non-specific epitope mapping of avian hepatitis E virus capsid protein for differential diagnosis of hepatitis E virus infection. Conference of Research Workers in Animal Diseases, Chicago, IL, November 14-16, 2004.
6. Zhou E-M*. A monoclonal anti-idiotypic antibody identified a putative cellular receptor for porcine reproductive and respiratory syndrome virus. Conference of Research Workers in Animal Diseases, Chicago, IL, November 14-16, 2004.
7. Kirby DK and Zhou E-M*. Evaluation of fluorescent focus neutralization assay in comparison with SVN, ELISA and IFA for serological diagnosis of porcine reproductive and respiratory syndrome virus infection. 47th Annual Conference of American Association of Veterinary Laboratory Diagnosticians. Greensboro, NC, October 21-25, 2004.
8. Zhou E-M*, Kirby DK, Lin K, Reynolds, DL, Fabios J, Platt, KB, Bogenschutz T, Garner, DL. Evaluation of a blocking ELISA for detection of West Nile virus antibodies in horses, chickens and wild ring-necked pheasants. The 18th Congress of the International Pig Veterinary Society, Hamburg, Germany, June 27-July 1, 2004.
9. Zhou E-M*. Identification of a putative PRRS virus receptor by a monoclonal anti-idiotype. The 18th Congress of the International Pig Veterinary Society, Hamburg, Germany, June 27-July 1, 2004.
10. Baldwin CJ, Schreiner CA, Zhou E-M and Evans RB. Seroprevalence of Leptospirosis in Midwest dogs. American College of Veterinary Internal Medicine. June 2004.
11. Ameri-Mahabadi M and Zhou E-M*. Western Blot Immunoassay as a Confirmatory Test for ELISAs to Detect Antibodies against Mycoplasma hyopneumoniae. 46th Annual AAVLD Meeting, San Diego, CA 2003.
12. Skibbe D, Dunaway S and Zhou E-M*. Further evaluation of the SIV H1N1 ELISA for serodiagnosis of SIV. North Central Conference of Veterinary Laboratory Diagnosticians, June 9-10, 2003, Minneapolis-St. Paul, MN.
13. Ameri-Mahabadi M and Zhou E-M*. Diagnosis of swine Actinobacillus pleuropneumoniae by using serological techniques. North Central Conference of Veterinary Laboratory Diagnosticians, June 9-10, 2003, Minneapolis-St. Paul, MN.
14. McKean JD, Beary J, Brockus S, O’Connor AM and Zhou E-M. Determination of Toxoplasma gondii antibody prevalence in Midwest market swine. Salinpork Program Conference, September 2003.
15. O’Connor AM, McKean JD, Beary J, Brockus S and Zhou E-M. The sero-prevalence of Salmonella spp. In finishing swine in Iowa. 5th International Symposium on the Epidemiology and Control of Foodborne Pathogens of Pork. 2003.
16. Pyburn DG, Patton S, Zimmerman JJ, McKean JD, Evans RB, O’Connor AM, Smedley KL and Zhou E-M. Risk factors for swine infection with Toxoplasma gondii. Ames Zoonosis Meeting, April 28, 2003.
17. Zhou E-M*, Jiang Z-J and Zimmerman JJ. Auto-anti-idiotype: its potential regulation of porcine reproductive and respiratory syndrome virus infection. Experimental Biology 2002. The FASEB Journal, 518.18.
18. Ameri-Mahabadi M and Zhou E-M*. Serodiagnosis of Swine Actinobacillus pleuropneuomoniae: Evaluation of An Indirect ELISA in Comparison with Complement Fixation Test. 45th Annual AAVLD Meeting, St. Louis, MO, October 16-25, 2002.
19. Zhou E-M*. Serodiagnosis of swine Mycoplasma hyopneumoniae: Evaluation of current commercial ELISA tests and development of alternative serologic assays. 14th International Congress of the International Organization for Mycoplasmology, July 7-12, 2002, Vienna, Austria.
20. Zhou E-M*. Evaluation of a blocking ELISA for serodiagnosis of PRRS. International Pig Veterinary Society 2002, June 2 –5, 2002, Ames, Iowa.
21. Ameri-Mahabadi M and Zhou E-M*. Comparison of Two Commercial ELISAs for Serodiagnosis of Swine Mycoplasma hyopneumoniae. North Central Conference of Veterinary Laboratory Diagnosticians, June 10-11, 2002, Reynoldsburg, OH.
22. Skibbe D, Dunaway S and Zhou E-M*. Evaluation of the Swine Influenza Virus Antibody Test Kit-H1N1 by IDEXX by comparison with Hemagglutination Inhibition of H1N1 and H3N2. North Central Conference of Veterinary Laboratory Diagnosticians, June 10-11, 2002, Reynoldsburg, OH.
23. Zhou E-M*, Zheng J and Zimmerman JJ. Auto-Anti-Idiotype: Its potential regulation of porcine reproductive and respiratory syndrome virus infection. Annual Meeting of the American Association of Immunologists, Experimental Biology, April 20-24, 2002, New Orleans, LA.
24. Skibbe D, Etter D, Ramirez M and Zhou E-M*. PRRSV antigen production in different cell lines. 40th Annual North Central Conference of Veterinary Laboratory Diagnosticians, June 11-12, 2001. University of Illinois at Urbana-Champaign.
25. Ramirez M, Norris S and Zhou E-M*. Evaluation of two commercial Johne’s ELISA kits. 40th Annual North Central Conference of Veterinary Laboratory Diagnosticians, June 11-12, 2001. University of Illinois at Urbana-Champaign.
26. Zhou E-M*, Zimmerman JJ, Zhou KX, Platt KB, Yoon KJ and Horter D. Development of A Blocking ELISA for Detection of Swine Antibodies to PRRSV. 44th Annual Meeting of American Association of Veterinary Laboratory Diagnosticians, Hershey PA, Nov.1-8, 2001.
27. Clavijo A, Riva J, Zhou E-M, Mallory M, Lin F and Lin M. Development of a recombinant protein-based competitive-ELISA for detection of antibodies to classical swine fever virus. Pestivirus Infection of Swine: Impact, Detection, Prevention and Control. Iowa State University, College of Veterinary Medicine, Ames, Iowa. July 6-7, 2000. Sponsored by NADC, PIADC, NVSL and ISU.
28. Barrandeguy M, Zhou E-M, Pacheco J, Costantini V, Craig MI, Ricci L and Fernandez F. Diagnostico Rapido De Influenza Equina Por ELISA. XVII Renuion Cientifica Anual. Bueno Aires, Argentina, Dec. 1997.
29. Lin M and Zhou E-M*. Evidence that two distinct populations of rabbit anti-idiotypic antibodies induced by three monoclonal antibodies specific for bluetongue virus core protein VP7. Canadian Society for Immunology Spring 95' Meeting. Lake Louise, Alberta, Canada, March 24-27, 1995.
30. Zhou E-M*, Huang W and Lin M. Anti-idiotype as serodiagnostic reagent for bluetongue virus infection. IV International Veterinary Immunology Symposium. University of California, Davis, USA, July 16-21, 1995.
31. Zhou E-M*. Development of novel anti-idiotype diagnostic reagent and vaccine against pseudorabies virus gp50. IV International Veterinary Immunology Symposium. University of California, Davis, USA, July 16-21, 1995.
32. Xu G, Wilson WC, Zhou E-M and Tabachnick WJ. Role of bluetongue virus VP7 protein on membrane binding in the insect vector, Culicoides variipenis. ESA Annual Meeting, Las Vegas, NV December 17-21, 1995.
33. Zhou E-M*, Afshar A, Heckert RA and Nielsen K. Anti-idiotypic antibodies generated by sequential immunization detect the shared audiotape on antibodies to pseudorabies virus antigens. Canadian Society for Immunology Spring '94 Meeting. Sainte-Adèle, Québec, March 11-14, 1994.
34. Prud'homme I, Traykova M, Zhou E-M and Harding MJ. Development of a baculovirus-derived Aujeszky's disease viral antigen. The IVth International Conference of Veterinary Virology. Switzerland, Sept. 1994.
35. Zhou E-M* and Kisil FT. The internal image anti-idiotypic antibody modulates the formation of antibodies to Ryegrass pollen allergen Lol pIV. The 48th Annual Meeting of American Academy of Allergy and Immunology, Orlando, FL, March 6 - March 11, 1992.
36. Zhou E-M* and Kisil FT. Suppression of the formation of antibodies to allergen Lol pIV could be adoptively transferred to the syngeneic recipients by the anti-idiotypic antibody-treated B lymphocytes. The 92`s Spring Meeting of Canadian Society of Immunology, Montreal, March 13 March 16, 1992.
37. Zhou E-M* and Kisil FT. The internal image anti-idiotypic antibody induces the anti-anti-idiotypic antibodies that are specific for Ryegrass pollen allergen Lol pIV. The 8th International Congress of Immunology, Budapest, Hungary, August 23 - August 28, 1992.
38. Zhou E-M* and Kisil FT. Regulation of antibody formation to Ryegrass pollen allergen Lol pIV by an internal image anti-idiotypic monoclonal antibody. The 47th Annual Meeting of American Academy of Allergy and Immunology, San Francisco, CA, March 1 - March 6, 1991.
39. Zhou E-M*, Kisil FT, Dzuba-Fisher JMM, Rector ES and Sehon AH. A murine monoclonal anti-idiotypic antibody raised against a monoclonal antibody to Ryegrass pollen allergen Lol pIV detects a common idiotope on human, mouse, and rabbit antibodies to Lol pIV. The 46th Annual Meeting of American Academy of Allergy and Immunology, Maryland, March 23 - March 28, 1990.
40. Zhou E-M, Kanda P and Kennedy RC. Administration of monoclonal anti-idiotypic antibody induces silent idiotypes that bind human immunodeficiency virus envelope glycoprotein epitopes. The 72th Annual Meeting of Federation of American Societies for Experimental Biology, Los Vegas, NV, May 1 - 5, 1988.
41. Zhou E-M, Chanh TC and Kennedy RC. Mouse monoclonal anti-anti-CD4 antibodies recognize human immunodeficiency virus antigens. The 71th Annual Meeting of Federation of American Societies for Experimental Biology, Washington, D.C., March 29 - April 2, 1987.
42. Kennedy RC, Zhou E-M, Dreesman GR and Chanh TC. Internal image anti-idiotypes representative of homobodies mimic the CD4 molecule and bind human immunodeficiency virus. The III International Congress on AIDS, Washington, D.C., 1987.
43. Chanh TC, Alderete BE, Zhou E-M and Kennedy RC. Anti-idiotypic antibodies against OKT4A bind to human immunodeficiency virus. The 71th Annual Meeting of Federation of American Societies for Experimental Biology, Washington, D.C., March 29 - April 2, 1987.
STANDARD OPERATION PROCEDURES（SOP）
1. Immunoblot Assay as C-ELISA Confirmatory Test of Antibodies against Bluetongue Virus Core Protein VP7. Agriculture Canada, Animal and Plant Health Division Standard Protocol Version 1.0, 1995.
2. Avian Influenza: Competitive Enzyme Immunoassay for the Detection of Antibodies to Avian Influenza Virus. Agriculture Canada, Animal and Plant Health Division Standard Protocol Version 1.0, 1996.
3. Epizootic Hemorrhagic Disease of Deer: Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to EHD Virus. Agriculture Canada, Animal and Plant Health Division Standard Protocol Version 1.0, 1996.
4. Production of Recombinant NP Antigen of Type A Influenza Virus in Insect Cell Culture. Agriculture Canada, Animal and Plant Health Division Standard Protocol Version 1.0, 1997.
5. Bluetongue (BT): Agar Gel Immunodiffusion (AGID) Test for the Detection of Antibodies to BT Virus. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 2.0, 2000.
6. Equine Infectious Anemia (EIA): Agar Gel Immunodiffusion (AGID) Test for the Detection of Antibodies to EIA Virus. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0, 2000.
7. Electronic Report of Serology Test Results via VisuaLab. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0, 2002.
8. Mycoplasma hyopneumoniae: Indirect Enzyme-Linked Immunosorbent Assay for the Detection of Swine Antibodies to Mycoplasma hyopneumoniae. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0, 2000.
9. Immunoblotting Assay as ELISA Confirmatory Test of antibodies against Mycoplasma hyopneumoniae. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2003.
10. Porcine Parvovirus (PPV): Hemagglutination Inhibition Test for the Detection of Antibodies to PPV. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2000.
11. Swine Influenza: Hemagglutination Inhibition Test for the Detection of Antibodies to Swine Influenza Virus. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2000.
12. Pseudorabies Virus (PRV) (Aujesky’s Disease): Neutralization (SN) Test for the Detection of Swine Antibodies to PRV. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 2.0, 2000.
13. Porcine Circovirus II (PCV) Indirect Fluorescent Antibody (IFA) Procedure. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2003.
14. Caprine Arthritis - Encephlitis/Ovine Progressive Pneumonia Agar Gel Immunodiffusion (AGID) Assay for the Detection of Antibodies to CAE/OPP Virus. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
15. Johne’s Disease: Agar Gel Immunodiffusion (AGID) Assay for the Detection of Antibodies to Mycobacterium avium subspecies paratuberculosis in Sheep and Goats. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 2.0.2002.
16. Virus Neutralization Test for Bovine Respiratory Syncytial (BRSV). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
17. Virus Neutralization Test for Bovine Viral Diarrhea - Type I (BVD - I). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. April 2001. .
18. Virus Neutralization Test for Bovine Viral Diarrhea – Type II (BVD - II). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
19. Virus Neutralization Test for Para – Influenza III virus (PI 3). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
20. Virus Neutralization Test for Transmissible Gastroenteritis (TGE). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0.2001.
21. Virus Neutralization Test for the Detection of Antibodies to Canine Herpesvirus (CHV). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
22. Virus Neutralization Test for the Detection of Antibodies to Encephalomyocarditis (EMC). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
23. Virus Neutralization Test for Vesicular Stomatitis - Indiana Strain. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
24. Virus Neutralization Test for Vesicular Stomatitis – New Jersey Strain. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
25. Laboratory Standard Operating Procedure for the Receiving and Processing of Serology Samples. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0.2001.
26. Microscopic Agglutination test for Detecting Antibody to Leptospira interrogans in Animal Sera. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2001.
27. Propagation and Maintenance of Mammalian Cell Lines. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2002.
28. Bovine Leukemia: Agar Gel Immunodiffusion (AGID) Assay for the Detection of Antibodies to Bovine Leukemia Virus (BLV). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0.2001.
29. Hybridoma Media Preparation Procedure. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0.2003.
30. Cryopreservation Protocol: Myeloma and Hybridoma Cell Lines. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2003.
31. Labeling of Buffer Solutions for Serological Assays. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2003.
32. Isolation of Yolk from Quail Eggs for use in the detection of Avian Influenza. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0.2003.
33. PRRS fluorescent focus neutralization (FFN). Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2003.
34. Visitor Policy – Serology Section, VDL-ISU. Iowa State University, Veterinary Diagnostic Laboratory, Serology Section Standard Protocol Version 1.0. 2003.
INVITED LECTURES TO PEERS:
1. PRRS Immunology and Control and Practice at Western World. 2008 Hubei Area Swine Producers Summit, organized by Boehringer-Ingelheim, Wuhan, China, August 10-12, 2008.
2. Current Strategies for Prevention and Control of PRRS. Central China Swine Disease Prevention and Control Symposium, organized by Boehringer-Ingelheim, Zhengzhou, China, September 9-11, 2007.
3. Novel approaches for PRRS diagnosis: identification of PRRS antibodies in saliva and meat juice. The 13th Swine Disease Conference, Ames, IA, November 10-11, 2005.
4. Anti-idiotypic antibodies as a novel diagnostic reagent and potential vaccine for porcine reproductive and respiratory syndrome virus. National Quarantine Institute, Chinese Ministry of Agriculture, Qingdao, China, June 2003.
5. Immune modulation of PRRS virus infection and development of a novel diagnostic test for Mhyo antibody detection. Iowa Pork Congress, Des Moines, Iowa, January 2003.
6. How to Determine False Positive Reactions of PRRS ELISA. The 11th Iowa Swine Disease Conference, Ames, Iowa, November 2002.
7. IgM-capture ELISA as a serodiagnsotic test for detection of primary infection of PRRSV in pigs Annual Meeting of the American Association of Swine Veterinarians, March 2-5, 2002, Kansas City, Missouri.
8. Laboratory Diagnosis of pseudorabies at VDL, ISU. At Final Steps to Eradicating Pseudorabies from Iowa, Veterinary PRV Certification Training to 5 districts in Iowa. Iowa Department of Agriculture, Iowa Pork Producers Assoc., USDA APHIS Veterinary Services. Oct.16-18, 2001.
9. Anti-idiotype technique: an alternative approach for immunodiagnosis. 5th International Veterinary Immunology Symposium, Ludhiana, India, November, 8-13, 1998.
10. Competitive ELISA for Avian Influenza Serologic Surveillance. Fourth International Symposium on Avian Influenza. Athens, Georgia, May 28-31, 1997.
CONFERENCE AND MEETING PRESENTATIONS TO PEERS:
Oct. 2003 Western Blot Immunoassay as a Confirmatory Test for ELISAs to Detect Antibodies against Mycoplasma hyopneumoniae. American Association of Veterinary Laboratory Diagnosticians, San Diego, CA.
June 2003 Further evaluation of the SIV H1N1 ELISA for serodiagnosis of SIV. North Central Conference of Veterinary Laboratory Diagnosticians, Minneapolis-St. Paul, MN.
June 2003 Diagnosis of swine Actinobacillus pleuropneumoniae by using serological techniques. North Central Conference of Veterinary Laboratory Diagnosticians, Minneapolis-St. Paul, MN.
March 2003 Improve Immunodiagnostic Sensitivity and Specificity of PRRS Virus Infection and Identify Possible Potential Virus Carriers Using the Combined Immunological Assays. American Association of Swine Veterinarians, Kissimmee, Florida.
Oct. 2002 Serodiagnosis of Swine Actinobacillus pleuropneuomoniae: Evaluation of An Indirect ELISA in Comparison with Complement Fixation Test. American Association of Veterinary Laboratory Diagnosticians, St. Louis, MO.
July 2002 Serodiagnosis of swine Mycoplasma hyopneumoniae: Evaluation of current commercial ELISA tests and development of alternative serologic assays. 14th International Congress of the International Organization for Mycoplasmology, Vienna, Austria.
June 2002 Evaluation of a blocking ELISA for serodiagnosis of PRRS, International Pig Veterinary Society 2002, Ames, Iowa.
June 2002 Comparison of Two Commercial ELISAs for Serodiagnosis of Swine Mycoplasma hyopneumoniae. North Central Conference of Veterinary Laboratory Diagnosticians, Reynoldsburg, OH.
June 2002 Evaluation of the Swine Influenza Virus Antibody Test Kit-H1N1 by IDEXX by comparison with Hemagglutination Inhibition of H1N1 and H3N2. North Central Conference of Veterinary Laboratory Diagnosticians, Reynoldsburg, OH.
March 2002 IgM-capture ELISA as a serodiagnostic test for detection of primary infection of PRRSV in pigs. 33rd Annual Meeting of American Association of Swine Veterinarians, Kansas City, Missouri.
Feb. 2002 Validation of ELISA-gpI test for use with meat juice. Meat juice PRV surveillance meeting, USDA-APHIS, Ames, Iowa.
Nov. 2001 Development of A Blocking ELISA for Detection of Swine Antibodies to PRRSV. 44th Annual Meeting of American Association of Veterinary Laboratory Diagnosticians, Hershey PA.
June 2001 PRRSV antigen production in different cell lines. North Central Conference of Veterinary Laboratory Diagnosticians, Urbana-Champaign, Wisconsin.
June 2001 Evaluation of two commercial Johne’s ELISA kits. 40th Annual North Central Conference of Veterinary Laboratory Diagnosticians, Urbana-Champaign, Wisconsin.
Nov. 1998 Anti-idiotype technique: an alternative approach for immunodiagnosis. Fifth International Veterinary Symposium, Ludhiana, India.
May 1997 Competitive ELISA for Avian Influenza Serologic Surveillance. Fourth International Symposium on Avian Influenza, Athens, Georgia.
July 1995 Anti-idiotype as serodiagnostic reagent for bluetongue virus infection. IV International Veterinary Immunology Symposium. Davis, CA.
Oct. 1994 Anti-idiotypic antibodies as potential candidates of vaccines and serodiagnostic reagents to infectious diseases. IBC International Symposium of Veterinary Vaccines. Bethesda, MD.
June 1993 Anti-idiotype: Their potential in serological testing for foreign animal diseases. Abstract in the 43rd Conference of Canadian Laboratory Workers in Animal Diseases, Nepean, Ontario, Canada.
PRESENTATIONS TO PRODUCERS AND COMMODITY GROUPS:
August 2003 Evaluation of serologic diagnosis of swine influenza H1N1 to detect antibodies against SIV H1N1 Pfizer vaccine strain. Ames, Iowa.
April 2003 Improvement of swine influenza serodiagnosis for specific detection of antibodies against Pfizer’s SIV vaccine strain. Ames, Iowa.
January 2003 Immune modulation of PRRS virus infection and development of a novel diagnostic test for Mhyo antibody detection. Iowa Pork Board, Des Moines, Iowa.
Oct. 2001 Laboratory Diagnosis of pseudorabies at VDL, ISU. At Final Steps to Eradicating Pseudorabies from Iowa, Veterinary PRV Certification Training to 5 districts in Iowa. Organized by Iowa Department of Agriculture, Iowa Pork Producers Assoc., USDA APHIS Veterinary Services
Dec. 2000 Enzyme-linked immunosorbent assay (ELISA): a serological diagnostic tool for detection of antibodies. Descriptive paper written for the clients of ISU’s VDL.
BOARD OF SCIENTIFIC REVIEWERS:
Transboundary and Emerging Disease: 2007-present.
American Journal of Veterinary Research: 2002-2007.
JOURNAL REFEREE:
American Journal of Veterinary Research
Journal of Diary Research
Journal of Veterinary Diagnostic Investigation
Journal of Virological Methods
Veterinary Microbiology
Viral Immunology
SERVICE IN PROFESSIONAL SOCIETIES:
Member of Council of Veterinary Publish Health, CAAV, 2007- present
Member of Chinese Association for Immunology, 2007- present.
Member of American Veterinary Immunologist, 1996-2006
Member of American Association of Veterinary Laboratory Diagnosticians, 2000-2006
Member of American Society for Microbiology, 2002-2006
Member of National Institute for Animal Agriculture, 2002-2006
Cattle Health Committee member
Emerging Diseases Committee member
Member of Canadian Society for Immunology, 1996-2000
INSTITUTIONAL SERVICE:
Northwest A&F Univeristy
Academic Degree Committee (the Ninth Term) members, 2010-
Responsibilities: for approval of BS, MS and PhD degree.
Scientific Committee (the Third Term) member, 2010-2013
Responsibilities: for university’s development, research and scientific communication
Lowa State University
Premium for Academic Excellence (PACE) Awards Committee, 2004-2006.
Responsibilities: to review ISU student application for PACE awards about 30 applications each year and make recommendation to Graduate College.
Member of VDL Safety Committee, 2003-2006.
Responsibilities: Develop protocols and policies for safety related subjects.
Member of Graduate Faculty Term Membership Committee, 2001-2004.
Responsibilities: to review faculty applicants for the Graduate Faculty Term Membership. The committee members first preview the applicants and then meet to review make recommendation to Dean of the Graduate College whether accept or reject the applicant to become Graduate Faculty Term member. Typically, 30-40 applications reviewed each year.
Healthy Livestock Initiative Research Proposal Review Committee, 2004.
Responsibilities: Reviewed 4 proposals submitted for scientific merits and funding recommendations.
Member of VDPAM Planning Committee, 2002.
Responsibilities: The mission of the committee was to address the issues of 1) departmental organization and structure, 2) planning/vision/goals, 3) budget, 4) service to clients and excellence and 5) space and resources.
Canadian Food Inspection Agency (Agricultural Canada):
Member of the Scientific Review Committee for the Science, Advising and Management Division (SAMD) of Canadian Food Inspection Agency (CFIA), 1997-1998.
Responsibilities: Review and recommend for funding the grant proposals submitted by scientists working in CFIA nationwide. Typically, the committee reviewed 50-70 grant proposals each year.
Member in the ELISA Methods Program Committee, Animal Disease Research Institute (ADRI)/National Centre for Foreign Animal Disease, Canadian Food Inspection Agency (CFIA, 1995-1997.
Responsibilities: Develop a unified ELISA method program that was used for serodiagnosis of animal diseases.
Member of the Radiation Safety Committee, ADRI, 1995-1998.
Responsibilities: Develop protocols and policies for safe use of radioactive materials.
Member of the Scientific Review Committee for the Federal Laboratories Animal Care Committee (Health Canada and CFIA), 1998-1999.
Responsibilities: Develop protocols and policies for safe use of animals in research and diagnostic purposes and to review and prove the animal usage documents.